Journal: bioRxiv

Article Title: HTRA3 protease-chaperone stabilizes cathepsin B for mitochondrial POLG1 depletion in human cell ageing

doi: 10.1101/2025.07.21.647696

Figure Lengend Snippet: (A) WB of CSB, HTRA3 (left panel), and CTSB, and p21 (right panel) in BJ fibroblasts untreated and 10 days post-irradiation using ß-tubulin and GAPDH, respectively, as loading control. (B) Scheme showing the two hypotheses for the mechanism leading to senescence-related HTRA3 and CTSB overexpression. (C) Immunoblots of p21, CSB, HTRA3, CTSB, and POLG1 in BJ-5ta hTERT WT fibroblasts and 2 CRISPR-edited CSB knocked-out clones (BJ-5ta csb-/- hTERT #1 and BJ-5ta csb-/- hTERT #2). Frames show samples on the same blot; each displaying the respective GAPDH used as loading control. (D) Scheme indicating normally blocked senescence in BJ-5ta hTERT cells and activation of senescence upon irradiation at 10 Gy. WB analysis of (E) CSB, CTSB, p21, and (F) HTRA3 three days post-irradiation in BJ-5ta hTERT WT fibroblasts and BJ-5ta hTERT CSB knocked-out fibroblasts (β-tubulin was used as loading control). RT-qPCR of ( G ) p21 Waf1 and ( H ) HTRA3 and ( I ) CTSB in the same experiment and cells schematized in panel D. Samples on the same blot are framed; each frame displays the respective GAPDH or β-tubulin used as loading control. RT-qPCR: n=3 independent experiments; mean ± SD. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001; based on unpaired t test comparisons to respective non-irradiated control.

Article Snippet: Normal female human foetal lung IMR-90 fibroblasts (ATCC; CCL-186) and BJ skin fibroblasts (ATCC; CRL-2522) were cultured in minimum essential medium (MEM, Gibco) supplemented with 2 mM L-glutamin (GlutMAX), 10% FBS, 1% Penicillin–streptomycin, 1% nonessential amino acids (Gibco) and 1% sodium pyruvate (Gibco) BJ-5ta hTERT immortalized human fibroblasts (ATCC; CCL-186) cultured in a 4:1 mixture of DMEM and Medium 199 (Gibco) supplemented with 10% FBS and1% Penicillin–streptomycin.

Techniques: Irradiation, Control, Over Expression, Western Blot, CRISPR, Clone Assay, Activation Assay, Quantitative RT-PCR

Journal: Reproductive Sciences

Article Title: The Essential Role of GATA6 in the Activation of Estrogen Synthesis in Endometriosis

doi: 10.1177/1933719118756751

Figure Lengend Snippet: A, Differences in estradiol (E2) production in OSIS experimental groups. N = 4. Asterisk (*) indicates significantly lower concentration compared to other groups tested (P < .05). B, Differences in estradiol (E2) production in normal endometrial stromal cells (NoEMs) experimental groups. N = 4. Asterisk (*) indicates a significantly higher concentration compared to other groups tested (P < .05). C, Differences in estradiol (E2) production in skin fibroblast (BJ) experimental groups. N = 4.

Article Snippet: Human foreskin fibroblast cells (BJ cells), a nonimmortalized stromal cell line with a normal diploid karyotype, were obtained from ATCC and used as controls (cat. CRL-2522; Manassas, Virginia; n = 7).

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Orange Peel-Mediated Green Synthesis of ZnO and CuO Nanoparticles: Evaluation for Antimicrobial Activity and Biocompatibility in Tissue Engineering

doi: 10.3390/ijms26188781

Figure Lengend Snippet: Cytotoxicity assessment of green-synthesized CuO NPs after 4 days of incubation for ( a , c ) murine fibroblast L929 cells, and ( b , d ) MG-63 osteoblast-like cells. The negative control consisted of cells incubated only with complete culture medium. Data are expressed as mean ± SD, where n = 3. Statistical analysis was performed using Student’s t -test (** p ≤ 0.01, *** p ≤ 0.001).

Article Snippet: For cell viability assessment and cell culture preparation, immortalized murine fibroblast L929 cells, primary human dermal fibroblast BJ cells (both obtained from American Type Culture Collection, ATCC, Manassas, VA, USA), and MG-63 osteoblast-like cells (Cell Lines Service GmbH, Heidelberg, Germany) were maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% Penicillin-Streptomycin antibiotic solution.

Techniques: Synthesized, Incubation, Negative Control

Journal: International Journal of Molecular Sciences

Article Title: Orange Peel-Mediated Green Synthesis of ZnO and CuO Nanoparticles: Evaluation for Antimicrobial Activity and Biocompatibility in Tissue Engineering

doi: 10.3390/ijms26188781

Figure Lengend Snippet: Cytotoxicity assessment of green-synthesized ZnO NPs after 4 days of incubation for ( a , c ) murine fibroblast L929 cells, and ( b , d ) MG-63 osteoblast-like cells. The negative control consisted of cells incubated only with complete culture medium. Data are expressed as mean ± SD, where n = 3. Statistical analysis was performed using Student’s t -test (** p ≤ 0.01, *** p ≤ 0.001).

Article Snippet: For cell viability assessment and cell culture preparation, immortalized murine fibroblast L929 cells, primary human dermal fibroblast BJ cells (both obtained from American Type Culture Collection, ATCC, Manassas, VA, USA), and MG-63 osteoblast-like cells (Cell Lines Service GmbH, Heidelberg, Germany) were maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% Penicillin-Streptomycin antibiotic solution.

Techniques: Synthesized, Incubation, Negative Control